Dev097444 1660..1670

نویسندگان

  • Keiko Sakakibara
  • Pascal Reisewitz
  • Tsuyoshi Aoyama
  • Thomas Friedrich
  • Sayuri Ando
  • Yoshikatsu Sato
  • Yosuke Tamada
  • Tomoaki Nishiyama
  • Yuji Hiwatashi
  • Tetsuya Kurata
  • Masaki Ishikawa
  • Hironori Deguchi
  • Stefan A. Rensing
  • Wolfgang Werr
  • Takashi Murata
  • Mitsuyasu Hasebe
  • Thomas Laux
چکیده

Many differentiated plant cells can dedifferentiate into stem cells, reflecting the remarkable developmental plasticity of plants. In the moss Physcomitrella patens, cells at the wound margin of detached leaves become reprogrammed into stem cells. Here, we report that two paralogous P. patens WUSCHEL-related homeobox 13-like (PpWOX13L) genes, homologs of stem cell regulators in flowering plants, are transiently upregulated and required for the initiation of cell growth during stem cell formation. Concordantly, Δppwox13l deletion mutants fail to upregulate genes encoding homologs of cell wall loosening factors during this process. During themoss life cycle, most of the Δppwox13lmutant zygotes fail to expand and initiate an apical stem cell to form the embryo. Our data show that PpWOX13L genes are required for the initiation of cell growth specifically during stem cell formation, in analogy to WOX stem cell functions in seed plants, but using a different cellular mechanism.

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تاریخ انتشار 2014